目的 建立高效液相色谱法测定大腹皮中槟榔碱的含量。方法 采用Thermo-BioBasic SCX离子交换柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.2%磷酸溶液(55∶45),浓氨试液调节pH值至3.8,流速1.0 mL·min^-1,柱温30 ℃,检测波长215 nm。结果 氢溴酸槟榔碱的色谱峰面积与质量浓度呈良好的线性关系,线性范围为5.2~83.2 μg·mL^-1 (r=0.999 1),平均回收率(n= 9)为98.8%。 结论 该方法简便、准确、专属性强,为大腹皮饮片的质量评价提供了方法。

OBJECTIVE To establish a quantitative determination method for arecoline in Arecae Pericarpium. METHODS The determination was performed on thermo-biobasic SCX column (4.6 mm×250 mm, 5 μm) with mobile phase consisting of acetonitrile-phosphoric acid solution (55;45). The flow rate was 1.0 mL·min^-1, the column temperature was 30 ℃, and the detection wavelength was 215 nm. RESULTS The method had good linear relationship for arecoline hydrobromide in the range of 5.2-83.2 μg·mL^-1 (r=0.999 1). The average recovery (n=9) was 98.8% for arecoline hydrobromide. CONCLUSION The method is simple, accurate and specific. It provides a reliable way for evaluating the quality of Arecae Pericarpium.